Search results

On-demand Presentation

Protein Characterization with Light Scattering

General overview of different protein characterization applications. Brief introduction of the theory of static light scattering (SLS), dynamic light scattering (DLS), and electrophoretic light scattering (ELS). Protein melting point by dynamic light scattering is demonstrated with hemoglobin. Molecular shape can be estimated, when the absolute molecular weight (MW) is known. Molecular interactions (for example antibody-antigen binding) or formation of quaternary structure (as shown on insulin dimer / hexamer) can be studied. Static light scattering is used for the determination of the absolute molecular weight, and the second virial coefficient.

Type:	Dual Polarization Interferometry (DPI)
Duration:	7 minutes
Language:

Search for more On-demand Presentations: