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Protein Crystallization Screening

In protein structure determination x-ray protein crystallography is still the best tool available today. The bottleneck in determining protein structure remains the actual protein crystallization step and more specifically, the search for the optimum protein crystallization condition. Random screens of buffers are often applied to find a hit or narrow the search area. A different approach involves the measurement of the solvent-protein interactions via the second virial coefficient. If this parameter falls into a narrow "crystallization slot" conditions are ideal. However, this is time consuming. A faster snapshot of the protein in solution is obtained with dynamic light scattering. The width of the size distribution (polydispersity) is related to the chance of protein crystallization success. DLS provides a fast, non-invasive, low volume, sensitive screening technique for assessing crystallizability of protein solutions.

Type:	Dynamic Light Scattering (PCS)
Duration:	5 minutes
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